abstract：:Cannabinoid receptors (CBRs) belong to the G protein-coupled receptor superfamily, and activation of CBRs in salivary cells inhibits agonist-stimulated salivation and modifies saliva content. However, the role of different CBR subtypes in acinar cell physiology and in intracellular signalling remains unclear. Here, we...

journal_title：Journal of cell science

authors： Kopach O,Vats J,Netsyk O,Voitenko N,Irving A,Fedirko N

更新日期：2012-04-15 00:00:00

abstract：:Actin filaments assemble into diverse protrusive and contractile structures to provide force for a number of vital cellular processes. Stress fibers are contractile actomyosin bundles found in many cultured non-muscle cells, where they have a central role in cell adhesion and morphogenesis. Focal-adhesion-anchored str...

journal_title：Journal of cell science

authors： Tojkander S,Gateva G,Lappalainen P

更新日期：2012-04-15 00:00:00

abstract：:Neutrophil migration is vital for immunity and precedes effector functions such as pathogen killing. Here, we report that this process is regulated by the Rab27a GTPase, a protein known to control granule exocytosis. Rab27a-deficient (Rab27a KO) neutrophils exhibit migration defects in vitro and in vivo, and live-cell...

journal_title：Journal of cell science

authors： Singh RK,Liao W,Tracey-White D,Recchi C,Tolmachova T,Rankin SM,Hume AN,Seabra MC

更新日期：2012-04-01 00:00:00

abstract：:Gp78 (also known as AMFR and RNF45) is an E3 ubiquitin ligase that targets proteins for proteasomal degradation through endoplasmic reticulum (ER)-associated degradation (ERAD). In this study, we showed that gp78-mediated ubiquitylation is initiated in the peripheral ER. Substrate monoubiquitylation and gp78 CUE domai...

journal_title：Journal of cell science

authors： St-Pierre P,Dang T,Joshi B,Nabi IR

更新日期：2012-04-01 00:00:00

abstract：:Although it is well established that embryonic stem (ES) cells have the potential to differentiate into dopamine neurons, the molecular basis of this process, particularly the role of microRNAs (miRNAs), remains largely unknown. Here we report that miR-132 plays a key role in the differentiation of dopamine neurons by...

journal_title：Journal of cell science

authors： Yang D,Li T,Wang Y,Tang Y,Cui H,Tang Y,Zhang X,Chen D,Shen N,Le W

更新日期：2012-04-01 00:00:00

abstract：:Adaptive immunity, a vertebrate specialization, adds memory and exquisite specificity to the basic innate immune responses present in invertebrates while conserving metabolic resources. In adaptive immunity, antigenic challenge requires extremely rapid proliferation of rare antigen-specific lymphocytes to produce larg...

journal_title：Journal of cell science

authors： Cantor JM,Ginsberg MH

更新日期：2012-03-15 00:00:00

abstract：:The process of angiogenesis requires endothelial cells (ECs) to undergo profound changes in shape and polarity. Although this must involve remodelling of the EC cytoskeleton, little is known about this process or the proteins that control it. We used a co-culture assay of angiogenesis to examine the cytoskeleton of EC...

journal_title：Journal of cell science

authors： Hetheridge C,Scott AN,Swain RK,Copeland JW,Higgs HN,Bicknell R,Mellor H

更新日期：2012-03-15 00:00:00

abstract：:Epithelial junctions depend on intercellular interactions between β(1) subunits of the Na(+)/K(+)-ATPase molecules of neighboring cells. The interaction between dog and rat subunits is less effective than the interaction between two dog β(1) subunits, indicating the importance of species-specific regions for β(1)-β(1)...

journal_title：Journal of cell science

authors： Tokhtaeva E,Sachs G,Sun H,Dada LA,Sznajder JI,Vagin O

更新日期：2012-03-15 00:00:00

abstract：:The position of the nucleus is regulated in different developmental stages and cellular events. During polarization, the nucleus moves away from the future leading edge and this movement is required for proper cell migration. Nuclear movement requires the LINC complex components nesprin-2G and SUN2, which form transme...

journal_title：Journal of cell science

authors： Borrego-Pinto J,Jegou T,Osorio DS,Auradé F,Gorjánácz M,Koch B,Mattaj IW,Gomes ER

更新日期：2012-03-01 00:00:00

abstract：:Epithelial cell polarity is essential for animal development. The scaffold protein Bazooka (Baz/PAR-3) forms apical polarity landmarks to organize epithelial cells. However, it is unclear how Baz is recruited to the plasma membrane and how this is coupled with downstream effects. Baz contains an oligomerization domain...

journal_title：Journal of cell science

authors： McKinley RF,Yu CG,Harris TJ

更新日期：2012-03-01 00:00:00

abstract：:The complex formed by Rad9, Rad1 and Hus1 (9-1-1) protects against genomic instability by activating DNA damage checkpoint and DNA damage repair pathways, mainly in response to replication fork collapse and UV lesions. Here we compare the role of Rad9A (also known as Rad9) with the human paralogue Rad9B. Unlike Rad9A,...

journal_title：Journal of cell science

authors： Pérez-Castro AJ,Freire R

更新日期：2012-03-01 00:00:00

abstract：:The LEM domain (for lamina-associated polypeptide, emerin, MAN1 domain) defines a group of nuclear proteins that bind chromatin through interaction of the LEM motif with the conserved DNA crosslinking protein, barrier-to-autointegration factor (BAF). Here, we describe a LEM protein annotated in databases as 'Ankyrin r...

journal_title：Journal of cell science

authors： Brachner A,Braun J,Ghodgaonkar M,Castor D,Zlopasa L,Ehrlich V,Jiricny J,Gotzmann J,Knasmüller S,Foisner R

更新日期：2012-02-15 00:00:00

abstract：:Syntaxin 1C (STX1C), produced by alternative splicing of the stx1A gene, is a soluble syntaxin lacking a SNARE domain and a transmembrane domain. It is unclear how soluble syntaxin can control intracellular membrane trafficking. We found that STX1C affected microtubule (MT) dynamics through its tubulin-binding domain ...

journal_title：Journal of cell science

authors： Nakayama T,Kamiguchi H,Akagawa K

更新日期：2012-02-15 00:00:00

abstract：:It is becoming clear that mechanical stimuli are crucial factors in regulating the biology of the cell, but the short-term structural response of a cell to mechanical forces remains relatively poorly understood. We mechanically stimulated cells transiently expressing actin-EGFP with controlled forces (0-20 nN) in orde...

journal_title：Journal of cell science

authors： Guolla L,Bertrand M,Haase K,Pelling AE

更新日期：2012-02-01 00:00:00

abstract：:A defining feature of malignant tumor progression is cellular penetration through the basement membrane and interstitial matrices that separate various cellular compartments. Accumulating evidence supports the notion that invasive cells employ specialized structures termed invadopodia to breach these structural barrie...

journal_title：Journal of cell science

authors： Grass GD,Bratoeva M,Toole BP

更新日期：2012-02-01 00:00:00

abstract：:Mob1 is a component of both the mitotic exit network and Hippo pathway, being required for cytokinesis, control of cell proliferation and apoptosis. Cell division accuracy is crucial in maintaining cell ploidy and genomic stability and relies on the correct establishment of the cell division axis, which is under the c...

journal_title：Journal of cell science

authors： Tavares A,Gonçalves J,Florindo C,Tavares AA,Soares H

更新日期：2012-01-15 00:00:00

abstract：:Primary cilia are conserved organelles that play crucial roles as mechano- and chemosensors, as well as transducing signaling cascades. Consequently, ciliary dysfunction results in a broad range of phenotypes: the ciliopathies. Bardet-Biedl syndrome (BBS), a model ciliopathy, is caused by mutations in 16 known genes. ...

journal_title：Journal of cell science

authors： Gascue C,Tan PL,Cardenas-Rodriguez M,Libisch G,Fernandez-Calero T,Liu YP,Astrada S,Robello C,Naya H,Katsanis N,Badano JL

更新日期：2012-01-15 00:00:00

abstract：:Integrin and receptor tyrosine kinase signalling networks cooperate to regulate various biological functions. The molecular details underlying the integration of both signalling networks remain largely uncharacterized. Here we identify a signalling module composed of a fibronectin-α5β1-integrin-integrin-linked-kinase ...

journal_title：Journal of cell science

authors： Azimifar SB,Böttcher RT,Zanivan S,Grashoff C,Krüger M,Legate KR,Mann M,Fässler R

更新日期：2012-01-15 00:00:00

abstract：:The oligosaccharyltransferase (OST) complex catalyses the N-glycosylation of polypeptides entering the endoplasmic reticulum, a process essential for the productive folding and trafficking of many secretory and membrane proteins. In eukaryotes, the OST typically comprises a homologous catalytic STT3 subunit complexed ...

journal_title：Journal of cell science

authors： Roboti P,High S

更新日期：2012-01-01 00:00:00

abstract：:Membrane contact sites (MCSs), where the membranes of two organelles are closely apposed, are regions where small molecules such as lipids or calcium are exchanged between organelles. We have identified a conserved membrane-binding domain found exclusively in proteins at MCSs in Saccharomyces cerevisiae. The synaptota...

journal_title：Journal of cell science

authors： Toulmay A,Prinz WA

更新日期：2012-01-01 00:00:00

abstract：:Traction forces increase after microtubule depolymerization; however, the signaling mechanisms underlying this, in particular the dependence upon myosin II, remain unclear. We investigated the mechanism of traction force increase after nocodazole-induced microtubule depolymerization by applying traction force microsco...

journal_title：Journal of cell science

authors： Rape A,Guo WH,Wang YL

更新日期：2011-12-15 00:00:00

abstract：:Polypeptide chains synthesized by membrane-bound ribosomes are translocated through, and integrated into, the endoplasmic reticulum (ER) membrane by means of the protein translocation channel, the translocon. Positive charges on the nascent chain determine the orientation of the hydrophobic segment as it is inserted i...

journal_title：Journal of cell science

authors： Fujita H,Yamagishi M,Kida Y,Sakaguchi M

更新日期：2011-12-15 00:00:00

abstract：:Ring canals connecting Drosophila germline, follicle and imaginal disc cells provide direct contact of cytoplasm between cells. To date, little is known about the formation, structure, or function of the somatic ring canals present in follicle and imaginal disc cells. Here, we show by confocal and electron microscopy ...

journal_title：Journal of cell science

authors： Airoldi SJ,McLean PF,Shimada Y,Cooley L

更新日期：2011-12-01 00:00:00

abstract：:Collagen V is a regulatory fibril-forming collagen that forms heterotypic fibrils with collagen I. Deletion of collagen V in the mouse is associated with a lack of fibril assembly in the embryonic mesenchyme, with a resultant lethal phenotype. The current work elucidates the regulatory roles of collagen V during devel...

journal_title：Journal of cell science

authors： Sun M,Chen S,Adams SM,Florer JB,Liu H,Kao WW,Wenstrup RJ,Birk DE

更新日期：2011-12-01 00:00:00

abstract：:Accurate chromosome segregation requires the spindle assembly checkpoint to be active at the onset of mitosis, before being silenced following chromosome alignment. p31(comet) is a checkpoint antagonist in that its inhibition delays mitotic exit, whereas its overexpression overrides the checkpoint. How exactly p31(com...

journal_title：Journal of cell science

authors： Westhorpe FG,Tighe A,Lara-Gonzalez P,Taylor SS

更新日期：2011-11-15 00:00:00

abstract：:Myosin-X (Myo10) is an unconventional myosin with MyTH4-FERM domains that is best known for its striking localization to the tips of filopodia and its ability to induce filopodia. Although the head domain of Myo10 enables it to function as an actin-based motor, its tail contains binding sites for several molecules wit...

journal_title：Journal of cell science

authors： Kerber ML,Cheney RE

更新日期：2011-11-15 00:00:00

abstract：:The key read-out of Wnt signalling is a change in the transcriptional profile of the cell, which is driven by β-catenin. β-catenin levels are normally kept low by a phosphorylation event that is mediated by glycogen synthase kinase 3 (GSK3, α- and β-isoforms), which targets β-catenin for ubiquitylation and proteasomal...

journal_title：Journal of cell science

authors： Metcalfe C,Bienz M

更新日期：2011-11-01 00:00:00

abstract：:Accumulation of adipocytes and collagen type-I-producing cells (fibrosis) is observed in muscular dystrophies. The origin of these cells had been largely unknown, but recently we identified mesenchymal progenitors positive for platelet-derived growth factor receptor alpha (PDGFRα) as the origin of adipocytes in skelet...

journal_title：Journal of cell science

authors： Uezumi A,Ito T,Morikawa D,Shimizu N,Yoneda T,Segawa M,Yamaguchi M,Ogawa R,Matev MM,Miyagoe-Suzuki Y,Takeda S,Tsujikawa K,Tsuchida K,Yamamoto H,Fukada S

更新日期：2011-11-01 00:00:00

abstract：:TRF1 is a key component of the telomere-capping complex and binds double-strand telomeric DNA as homodimers. So far, it is not clear whether TRF1 dimerization coincides with its telomere binding or is actively controlled before it binds the telomere, and in the latter case, how this event might affect its telomere ass...

journal_title：Journal of cell science

authors： Meng L,Hsu JK,Zhu Q,Lin T,Tsai RY

更新日期：2011-11-01 00:00:00

abstract：:Despite observations that the lacrimal gland has been identified as the principal source of dimeric immunoglobulin A (dIgA) in tears, the mechanism used by lacrimal gland acinar cells (LGACs) to transcytose dIgA produced by interstitial plasma cells is not well-characterized. This study identifies a transcytotic pathw...

journal_title：Journal of cell science

authors： Xu S,Edman M,Kothawala MS,Sun G,Chiang L,Mircheff A,Zhu L,Okamoto C,Hamm-Alvarez S

更新日期：2011-10-15 00:00:00

abstract：:SARA, an early endosomal protein, plays a key role in TGFβ signalling, as it presents SMAD2 and SMAD3 for phosphorylation by the activated TGFβ receptors. Here, we show that ERBIN is a new SARA-interacting protein that can be recruited by SARA to early endosomes. ERBIN was recently shown to bind and segregate phosphor...

journal_title：Journal of cell science

authors： Sflomos G,Kostaras E,Panopoulou E,Pappas N,Kyrkou A,Politou AS,Fotsis T,Murphy C

更新日期：2011-10-01 00:00:00

abstract：:Laminins are heterotrimeric glycoproteins of the extracellular matrix that are secreted by epithelial cells and which are crucial for the normal structure and function of the basement membrane. We have generated a mouse harboring a conditional knockout of α3 laminin (Lama3(fl/fl)), one of the main laminin subunits in ...

journal_title：Journal of cell science

authors： Urich D,Eisenberg JL,Hamill KJ,Takawira D,Chiarella SE,Soberanes S,Gonzalez A,Koentgen F,Manghi T,Hopkinson SB,Misharin AV,Perlman H,Mutlu GM,Budinger GR,Jones JC

更新日期：2011-09-01 00:00:00

abstract：:Prox1 plays pivotal roles during embryonic lymphatic development and maintenance of adult lymphatic systems by modulating the expression of various lymphatic endothelial cell (LEC) markers, such as vascular endothelial growth factor receptor 3 (VEGFR3). However, the molecular mechanisms by which Prox1 transactivates i...

journal_title：Journal of cell science

authors： Yoshimatsu Y,Yamazaki T,Mihira H,Itoh T,Suehiro J,Yuki K,Harada K,Morikawa M,Iwata C,Minami T,Morishita Y,Kodama T,Miyazono K,Watabe T

更新日期：2011-08-15 00:00:00

abstract：:Barrier junctions prevent pathogen invasion and restrict paracellular leakage across epithelial sheets. To understand how one barrier junction, the septate junction (SJ), is regulated in vivo, we used fluorescence recovery after photobleaching (FRAP) to examine SJ protein dynamics in Drosophila. Most SJ-associated pro...

journal_title：Journal of cell science

authors： Oshima K,Fehon RG

更新日期：2011-08-15 00:00:00

abstract：:When migrating mesenchymal cells collide, they exhibit a 'contact inhibition of locomotion' response that results in reversal of their front-rear polarity by extension of a new leading edge, which enables their migration away from the opposing contacted cell. The critical cytoskeletal rearrangements underpinning these...

journal_title：Journal of cell science

authors： Kadir S,Astin JW,Tahtamouni L,Martin P,Nobes CD

更新日期：2011-08-01 00:00:00

abstract：:Notch signaling is pivotal for the morphogenesis and homeostasis of many tissues. We found that aberrant Notch activation in mouse neural-crest-derived periocular mesenchymal cells (POMCs), which contribute to the formation of corneal and eyelid stroma, results in blepharophimosis. Compound transgenic mice overexpress...

journal_title：Journal of cell science

authors： Zhang Y,Kao WW,Pelosi E,Schlessinger D,Liu CY

更新日期：2011-08-01 00:00:00

abstract：:Hyperthermia adversely affects cell structure and function, but also induces adaptive responses that allow cells to tolerate these stressful conditions. For example, heat-induced expression of the molecular chaperone protein HSP70 can prevent stress-induced cell death by inhibiting signaling pathways that lead to apop...

journal_title：Journal of cell science

authors： Simard JP,Reynolds DN,Kraguljac AP,Smith GS,Mosser DD

更新日期：2011-07-15 00:00:00

abstract：:CK2 is a serine/threonine kinase with many substrates, largely unknown modes of regulation and essential roles in mitotic progression. CK2α, a catalytic subunit of CK2, is phosphorylated in mitosis, and here we examine the effect of phosphorylation on CK2α localization. Using phosphospecific antibodies, we show that C...

journal_title：Journal of cell science

authors： St-Denis NA,Bailey ML,Parker EL,Vilk G,Litchfield DW

更新日期：2011-07-15 00:00:00

abstract：:Although endocytosis and exocytosis have been extensively studied in budding yeast, there have been relatively few investigations of these complex processes in the fission yeast Schizosaccharomyces pombe. Here we identify and characterize fission yeast Mug33, a novel Tea1-interacting protein, and show that Mug33 is in...

journal_title：Journal of cell science

authors： Snaith HA,Thompson J,Yates JR 3rd,Sawin KE

更新日期：2011-07-01 00:00:00

abstract：:The mixed lineage leukemia (MLL) proto-oncogenic protein is a histone-lysine N-methyltransferase that is produced by proteolytic cleavage and self-association of the respective functionally distinct subunits (MLL(N) and MLL(C)) to form a holocomplex involved in epigenetic transcriptional regulation. On the basis of st...

journal_title：Journal of cell science

authors： Yokoyama A,Ficara F,Murphy MJ,Meisel C,Naresh A,Kitabayashi I,Cleary ML

更新日期：2011-07-01 00:00:00